Dilution Samples: Need to make 200 uL of each dilution We are doing serial dilut

Question

Dilution Samples: Need to make 200 uL of each dilution We are doing serial dilutions: For milk- 1:100, 1:1000, 1:2000, 1:4000 and 1:8000 For first supernatant- 1:100, 1:500, 1:1000, and 1:2000 For Whey- 1:100, 1:200, 1:400 and 1:800 For column fractions- 1:5, 1:10, 1:20, 1:40, and 1:80 And it has to be 200 uL total in volume. I understand that for 1:100 you would use 2uL milk and 198 uL water. And then use 2uL of this and put it in 198 uL of volume for a 1:1000. I do not understand how much to use from the 1:1000 to do 1:2000. ETC.

Explanation / Answer

dilute 1 in 199 for 1:2000

0.5 in 199.5ul for 1:4000

0.25ul in 199.75 for 1:8000

If you are having trouble in pipetting double the volumes and use.

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