Below is a list of analytes in common matrices. Match the optimal extraction met

Question

Below is a list of analytes in common matrices. Match the optimal extraction method to the desired extraction. Liquid-liquid extraction Solid phase extraction with a non-polar absorbent (reverse phase) Solid phase extraction with a polar absorbent (normal phase) Solid phase extraction with an ion exchange resin Size exclusion chromatography Affinity chromatography with an antibody adsorbent _____ Caffeine in a brewed coffee sample (pH ~ 2). ________ Tetrahydrocannabinol (THC) in a 5 g sample of marijuana extract. __________Fluoroacetate (a poison) in a 10 mL blood sample from a murder victim. _________ Heavy petroleum oils from motorboats in 100 g of sediment/Water mixture from the bottom of a lake. _______ Peptidylglycine alpha-amylating monooxygenase, an enzyme involved in amide biosynthesis, from a cell extract ________ A protein weighing 50 kDa in a mixture of proteins weighing 10 kDa, 50 kDa, and 100 kDa

Explanation / Answer

a) Caffeine in a brewed coffee sample can be extracted bym

D) Solid phase extraction with an ion exchange resin

b) Tetrahydrocarbinol can be extracted by a

B) liquid-liquid extraction

c) Fluroacetate can be extracted with,

C) liquid-liquid extraction

d) Heavy petroleum oil from motorboats in sediment/water mixture from the bottom of a lake can be extracted with,

E) size exclusion chromatography

e) Peptidoglycine alpha-amylating monooxygenase, an enzyme involved in amide biosynthesis, from a cell extract can be extracted with,

Affinity chromatographywith an antibody adsorbent

f) A protein in a mixture of proteins can be extracted with,

E) size exclusion chrmotography

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