Below is a list of analytes in common matrices. Match the optimal extraction met

Question

Below is a list of analytes in common matrices. Match the optimal extraction method to the desired extraction. Liquid-liquid extraction Solid phase extraction with a non-polar absorbent (reverse phase) Solid phase extraction with a polar absorbent (normal phase) Solid phase extraction with an ion exchange resin Size exclusion chromatography Affinity chromatography with an antibody adsorbent _________ Antillatoxin, a lipid-soluble lipopeptidic neutroxin, from a Water sample from a local contaminated stream __________ Aspirin (see structure below) in drinking Water samples from groundWater wells. The aspirin will be present at very low concentrations. _________ Estrogen (see structure below) in the sewage treatment plant outfall. __________ Alginate lyase, an enzyme involved in the processing of bacterial mucous, from a cell homogenate (i.e. mixture of proteins, DNA, lipids, and other metabolites) _______ Diethyl phthalate in the Water contained within a plastic drinking Water bottle. The concentrations are going to be low and laboratory contamination a serious problem. __________ synthetically made oxytocin, a hormone, from an acidified carbonic acid solution ________ As a first step in protein purification to remove very large small proteins

Explanation / Answer

a) Liquid liquid extraction

b) Solid phase extraction with polar absrobent

c) Solid phase extraction with nonpolar absrobent

d) Solid phase extraction with nonpolar absrobent

f) solid phase extraction with ion exchanger

g) size exclustion chromatography

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