Which of the following statements are true if the particle size in a packed colu
ID: 926114 • Letter: W
Question
Which of the following statements are true if the particle size in a packed column used in HPLC is decreased? (check all possible)
(a) Increased separation efficiency
(b) Requires a higher operating pressure
(c) Decreased operating temperatures
(d) Longer analysis times
(e) Allows the use of a lower operating pressure
Hints:
As the column particle size is decreased, the plate height will decrease with increased flow rate. Both separation efficiency and resolution are inversely proportional to plate height. Increased pressure is required to push the mobile phase through a packed column with a smaller particle size, which can result in frictional heating. From the given list, there are two correct answers.
Explanation / Answer
As the column particle size is decreased, the plate height will decrease.
And as plate height decreases separation efficiency will increases.
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Increased pressure is required to push the mobile phase through a packed column with a smaller particle size.
Therefore the true statements are
(a) Increased separation efficiency
(b) Requires a higher operating pressure.
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