A wide gradient from 5% to 100% acetonitrile applied over 46.7 min is used to se

Question

A wide gradient from 5% to 100% acetonitrile applied over 46.7 min is used to separate a mixture of 12 compounds. The sample is injected at t = dwell time. The first compound elutes from the column at 19.0 min and the last compound elutes at 31.9 min. From this run, it was determined that gradient elution would be appropriate for the separation of this mixture of compounds. Based on these results, determine the appropriate starting and ending values for the percent acetonitrile in the solvent for the second run over the same gradient time.

From % to % acetonitrile

Explanation / Answer

ANSWER:

From first run the solvent ratio has been determined or the method has been developed. And this gradient was found approprate for the said mixture. (as per question) . Hence for every other run for this mixture the solvent ratio will be same.

from 5% acetonitrile, to 100% acetonitrile.

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