Promoter fine mapping analysis is used to determine where the promoter starts an
ID: 92644 • Letter: P
Question
Promoter fine mapping analysis is used to determine where the promoter starts and ends in a gene by deleting regions of DNA adjacent to and within the gene. You have a putative gene which you subject to promoter fine mapping analysis. You observe that transcriptional activity is still maintained when deletion from the 5' end reaches nucleotide +53, but is lost upon further deletion past +53. Activity is also maintained when deletion from the 3' end reaches nucleotide +83, but is lost upon further deletion past +83. Please speculate what gene family this putative gene belongs to and state which RNA polymerase is used for its transcription
Explanation / Answer
During promotor fine mapping analysis, transcriptional activity is maintained when deletion from the 5' end reaches nucleotide +53, but is lost upon further deletion past +53. it is also seen that the transcriptional activity is maintained when deletion from the 3' end reaches nucleotide +83, but is lost upon further deletion past +83. Therefore, the putative gene belongs to cistron gene family. Cistron is the largest element in a gene which encodes for a polypeptide during protein synthesis. This putative gene is a promotor sequence. The RNA polymerase II binds to this region and used for its transcription.
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