(Dr. Acevedo) During your second year of medical school, you are tasked with pur

Question

(Dr. Acevedo) During your second year of medical school, you are tasked with purifying a protein using a nickel NTA column (see picture below). After purification you run a check gel (because you are amazing and always run controls) and you detect that your protein is contaminated with a nucleic acid. How would you remove the contamination? A. Add DNase. Rerun sample through column. B. Add RNase. Rerun sample through column. C. Add protease. Rerun sample through column. D. A & B. E. None of the above. The contamination cannot be removed.

Explanation / Answer

a. Usually DNAse can be added to the purified sample to hydrolyze any DNA present in the sample.But sometimes it leads to the loss of small amounts of sample also.

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