Which of the following changes in the physical parameters of a chromatography co

Question

Which of the following changes in the physical parameters of a chromatography column would improve efficiency of separating closely-eluting compounds? Circle all that apply.

a) making the stationary phase particles smaller in diameter

            b) making the coating on the stationary phase thicker so it retains longer

            c) making the coating on the stationary phase more evenly distributed

            d) using "Zero-volume" connections and fittings between components and in the detector

e) make the column shorter so everything elutes faster

            f) make the diameter of the column narrower to minimize lateral diffusion

Explanation / Answer

a) making the stationary phase particles smaller in diameter

b) making the coating on the stationary phase thicker so it retains longer

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