In the following experiment, researchers isolated rat liver nuclei and then trea
ID: 93464 • Letter: I
Question
In the following experiment, researchers isolated rat liver nuclei and then treated them with a chemical which activities the transcription of a particular gene of interest for various times (30 min). They then treated these nuclei with an enzyme that cuts DNA (DNAsel). They were then run out on an agarose gel. The ladder in the middle demonstrates the different sizes of DNA fragments generated. a. Explain what is happening to the DNA over time. b. Explain why your answer is 'a' is able to occur?Explanation / Answer
The phosphate backbone of the DNA molecule is negatively charged, therefore when placed in an electric field, DNA fragments will migrate to the positively charged anode. Because DNA has a uniform mass/charge ratio, DNA molecules are separated by size within an agarose gel in a pattern such that the distance traveled is inversely proportional to the log of its molecular weight.Thus the small fragments will move faster than the larger fragments.
I think in diagram "a" the larger fragments have stayed at the top and the smaller fragments are at the bottom as these have moved fast.
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