Without a promoter sequence, no mRNA copies of the transferred gene can be made.

Question

Without a promoter sequence, no mRNA copies of the transferred gene can be made. If by some quirk of the bacterial genetic machinery an mRNA transcript could be made of the gene, it still would be unable to bind to the fMet-tRNA and the small subunit of the ribosome. The ribosome cannot spontaneously start translation just anywhere on an mRNA molecule; a start codon is required for translation to proceed. You order increased measures for removal of the bacterial biofilms, as well as new counter-measures to limit spread of the bacteria. Since these measures have been implemented, repeat inspections by the company’s sanitizing crew have shown that no new mixed biofilms have formed in nozzles. The company’s in-house laboratory results show that E. coli is still sensitive to its current concentration of sanitizing agents and shows signs of resistance. You review the daily reports and confirm the findings

The question now is, “Why did E. coli not become more resistant if it had acquired new genes from L. monocytogenes?” The lab results show that genetic material was transferred and new peptides were made. But was it a complete gene that was transferred, or a properly folded protein that was synthesized?

Transcription and Translation . Order the following steps from transcription through the initiation of translation.

A. RNA polymerase begins synthesizing RNA by creating a DNA bubble.

B. Terminator DNA sequences loosen the grip of RNA polymerase and rho causes the release of the DNA strand.

C. The smaller subunit of rRNA attaches at the ribosomal binding site on the mRNA strand.

D. fMET-tRNA binds at the start codon.

E. The larger subunit of rRNA is recruited.

Explanation / Answer

The order is:

A.RNA polymerase begins synthesising RNA by creating a DNA bubble

B.terminator DNA sequence loosen the grip of RNA polymerase and rho causes the release of DNA strand

C.the smaller subunit of rRNA attatches at the ribosomal binding site on the mRNA

D. fMET - tRNA binds at the start codon

E.The larger the subunit of rRNA is recruited

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