Sketching \"dirty ligation\" Draw a rough sketch (denote fragment sizes, gene lo

Question

Sketching "dirty ligation" Draw a rough sketch (denote fragment sizes, gene locations, and gene directions) of five common plasmids that could result in the “dirty” ligation that we performed. You must assume that not all of the enzyme reactions where 100% effective. Indicate which ones of these plasmids you would observe if performing not “dirty” ligation. (please draw out the sketches thank you so much)

Plating Expected Results Plating mixture Plate Expected Results A-1 No DNA LB A-2 No DNA LB+Amp B-3 Plasmid control LB+Amp+)X -gal LB+Amp C-4 Dephosphorylation Control Vector+ water Experimental ligation: vector and insert LB+Amp+X -gal D-5

Explanation / Answer

Insufficient data in the question.please provide correct map for pTUDbeta vector. It seems to me that the gene after CMV promoter should be amp gene. Again in the table no mention is made of Tet plates as well as Amp + Tet plates.

Thank you.

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