A clear example of a dideoxy sequencing gel is shown in Figure 1. Try reading it

Question

A clear example of a dideoxy sequencing gel is shown in Figure 1. Try reading it. As read from the bottom of the gel to the top, the sequence corresponds to the mRNA for a protein. Can you find the open reading frame in this sequence? What protein does it code for?

A clear example of a dideoxy sequencing gel is shown in Figure 1. Try reading it. As read from the bottom of the gel to the top, the sequence corresponds to the mRNA for a protein. Can you find the open reading frame in this sequence? What protein does it code for?

Explanation / Answer

Ans-

DNA sequence-TATAAACTGGACAACCAGTTCGAGCTGGTGTTCGTGGTCGGTTTTCAGAAGATCCTAACGCTGACGTACGTAGACAAGTTGATAGATGATGTGCATCGGCTGTTTCGAGACAAGT

RNA sequence- UAUAAACUGG ACAACCAGUU CGAGCUGGUG UUCGUGGUCG GUUUUCAGAA GAUCCUAACGCUGACGUACG UAGACAAGUU GAUAGAUGAU GUGCAUCGGC UGUUUCGAGA CAAGU

Protein translation-5' to 3' --Open reading frame 1- Y K L D N Q F E L V F V V G F Q K I L T L T Y V D K L I D D V H R L F R D K- No start codon in frame first

Frame 2-I N W T T S S S W C S W S V F R R S Stop R Stop R T Stop T S Stop Stop Met Met C I G C F E T S

frame3- Stop T G Q P V R A G V R G R F S E D P N A D V R R Q V D R Stop C A S A V S R Q

3'-5'-- Frame 1-T C L E T A D A H H L S T C L R T S A L G S S E N R P R T P A R T G C P V Y

Frame2--L V S K Q P Met H I I Y Q L V Y V R Q R Stop D L L K T D H E H Q L E L V V Q F I

frame3-L S R N S R C T S S I N L S T Y V S V R I F Stop K P T T N T S S N W L S S L

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