Briefly describe the effect of the following procedural errors on s thin-layer c

Question

Briefly describe the

effect of the following procedural errors on s thin-layer chromatogram

Briefly describe the effect of the following procedural errors on a thin-layer chromatogram. (a) Applying too large a sample when spotting the plate (b) Placing the spotted plate into a developing chamber containing an eluent level above the level of 5 (c) Allowing the plate to remain in the developing chamber until the eluent front reaches the top of (d) Removing the plate from the developing chamber when the eluent front has moved only half-way (e) Developing the plate in an uncovered chamber. the origin. the plate. up the plate. bongonhonone with sodiuum horohvdride

Explanation / Answer

(a)Too large a sample spot will be a concentrated spot and hence might land up with bad separation of the spot.

(b) The origin level containing the compound may wash away with the eluent and gets dissolved in eluent.

(c) Below spot might run up and merge with other spots.

(d) Separation maynot be good, some spot might contain multiple spots..

(e) solvent will evaporate and the polarity of the eluent will change.

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