You need 350 mi of 1X TBE buffer to fill the electrophoresis tank and 50 ml of 1

Question

You need 350 mi of 1X TBE buffer to fill the electrophoresis tank and 50 ml of 1% IQWBMl gel sokjbon You are given 10XTBE buffer and a vial with agarose powder Show calculations to make the required solutions (2m) How pH affects charge and thus the migration of a molecule during etectrophoreeia: if me ecuie has only 1 charge, then can calculate as shown above for the moiecole Alanine if a molecule has more than 1 tfXWbla group, then the NET CHARGE on the molecule will be calculated by oonsidenng the charges of each tomtAbl* group For eg. s the chsrge on each kxuaMI group at this pH (deDOtid* on, value of this group) After you calculate the charge of each group, what is the NET CHARGE? (JaUl charge after adding ail tttWAbla group charges?)

Explanation / Answer

if pH of the buffer is 5, then it is still acidic.

and hence, charge on the ionisable group at this pH are:

NH3+ become as such and will have charge +1

COOH- become COOH and will have charge 0.

and the net charge of the molecule at this pH become +1.

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