The data at right describe the binding of a ligand to a proteirn L] mM) ] is the

Question

The data at right describe the binding of a ligand to a proteirn L] mM) ] is the concentration of free ligand 0.08 | 0.12 0.17 0.23 0.36 0.42 0.53 0.58 0.62 0.68 0.06 is the fraction of ligand-binding sites on the protein that are occupied by the ligand (Note that some textbooks use terms like v or Y, instead of 8, to denote fractional saturation.) | 0 10 0.14 0.21 0.37 0.53 0.79 1.11 1.31 1.70 Answer parts (a) and (b), below (a) Which of the following graphs could be used to estimate Kd for the data? 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0 O A and E O A and D O B and D O B and E O C and E 0.0 05 1.0 1.5 20 25 3.0 3.5 4.0 45 5.0 5.5 6.0 65 7.0 below...

Explanation / Answer

The dissociation constant (Kd) gives information on the concentrations of the reactants at equilibrium. From the graph, half of the fractional saturation gives the Kd value.

Kd= 0.85mM.

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