The enzyme catalase catalyzes the decomposition of H2O2 to H2O and O2. Use the d

Question

The enzyme catalase catalyzes the decomposition of H2O2 to H2O and O2. Use the data in the table to the right to determine the values of Rmax (the maximum rate of the enzyme, often called the maximum velocity, Vmax) and KM (the Michaelis-Menten constant of the enzyme). Answer the three parts below.

a) If you were to plot this data to graphically determine Rmax and KM using a Lineweaver-Burk plot, what would you plot? If you would use the original data, enter the original value in the corresponding blank.

Explanation / Answer

Lineweaver-Burk is represented as

1/R= (KM/Rmax)1/S +1/Rmax

so a plot of 1/S =1/[H2O2] vs 1/R will have to be drawn.

1.0      1/[H2O2] =1                                        R for 1.38 as 1/1.38= 0.724

5.0     1/[H2O2] =1/5 =0.2                              R for 6 as 1/6= 01.67

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