You want to test the role of a particular Cys residue in an enzyme by mutating i

Question

You want to test the role of a particular Cys residue in an enzyme by mutating it to another amino acid and seeing its effect on function. For the mutation, you want to pick an amino acid that is most similar in size and shape. Which amino acid would you pick and why? Give the full name, the 3 letter code, AND the l letter code. What types of amino acids would you most expect to see on the inside of a folded globular protein? Explain. Consider a reaction that occurs in human cells with a Delta G = 5 kJ mol^-1. What mechanisms could make the reaction spontaneous? Explain.

Explanation / Answer

13) The amino acid which is similar in size and shape to cysteine is methionine. Both contain sulphur groups in their side chain. The three letter code for Methionine is Met and one letter code is M.

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