Three polypeptides, the sequences of which are represented below using the one-l
ID: 970685 • Letter: T
Question
Three polypeptides, the sequences of which are represented below using the one-letter code for their amino acids, are present in a mixture: (Table 3-1 for abbreviation and charge) GASKLHRGADLMHKFQKAAG B: PYGDEPLDEPEE C: S WKGMHAAIV SLLFV Of the three, which one would elute last from an ion-exchange resin; beads coated with positively charged groups? an ion-exchange resin; beads coated with negatively charged groups? a size-exclusion (get-filtration) column designed to separate small peptides such as these?Explanation / Answer
(i) B would elute last
(ii) A would elute last
(iii) B would elute last
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