How would you design an experiment to demonstrate that the NLS (nuclear localiza

Question

How would you design an experiment to demonstrate that the NLS (nuclear localization signal) of the yeast protein Swi5 is sufficient for the transport of this protein into the nucleus?

A. Alter the sequence of Swi5 so that the NLS is deleted and demonstrate that the altered Swi5 protein cannot be detected in the nucleus.

B. Create a fusion between the NLS of Swi5 and a gene that is not normally found in the nucleus and demonstrate that this new fusion protein is now detected in the nucleus.

C. Compare the sequences of all of the known nuclear proteins in yeast and identify the amino acid sequence that is common to all of them.

D. Demonstrate that the NLS can bind importin .

E. Either experiment A or B would demonstrate that the NLS of Swi5 is sufficient for nuclear transport.

A. Alter the sequence of Swi5 so that the NLS is deleted and demonstrate that the altered Swi5 protein cannot be detected in the nucleus.

B. Create a fusion between the NLS of Swi5 and a gene that is not normally found in the nucleus and demonstrate that this new fusion protein is now detected in the nucleus.

C. Compare the sequences of all of the known nuclear proteins in yeast and identify the amino acid sequence that is common to all of them.

D. Demonstrate that the NLS can bind importin .

E. Either experiment A or B would demonstrate that the NLS of Swi5 is sufficient for nuclear transport.

Explanation / Answer

Create a fusion between the NLS of Swi5 and a gene that is not normally found in the nucleus and demonstrate that this new fusion protein is now detected in the nucleus. (expt A)

Alter the sequence of Swi5 so that the NLS is deleted and demonstrate that the altered Swi5 protein cannot be detected in the nucleus. (expt B)

Related Questions

Navigate

• Browse (All)
• Browse H
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.