You run an ELISA for molecule X. You test your sample, a negative control, and a

Question

You run an ELISA for molecule X. You test your sample, a negative control, and a positive control. How would you interpret the following data? Positive control: The ELISA turns dark blue Negative control: The ELISA turns dark blue Sample: The ELISA turns dark blue The sample is contaminated. The sample is negative for molecule X. The sample is positive for molecule X. You cannot accurately interpret these data, and you need to rerun the ELISA. You have a serum sample from a potential HIV patient that you are asked to test by ELISA Somehow you accidentally contaminate your sample with tryptic soy broth (a broth medium often used to grow bacteria) How will this change in the sample affect your ELISA? The ELISA will lose the ability to detect HIV It will make the concentration of HIV particles, if any, appear more than it really is. Otherwise the ELISA, should still work. It will make the concentration of HIV particles if any appear less than it really is. Otherwise the ELISA should still work. It would not affect the ELISA since this assay relies on very specie antibodies.

Explanation / Answer

Ans- part g- in this case the results can' be interpreted accurately so the ELISA should be rerun.

Part h- this would not effect the ELISA because ELISA is based on specific antibodies.

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