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A microarray assay (DNA chip), complementary DNA has been made from mRNA of seve

ID: 97901 • Letter: A

Question

A microarray assay (DNA chip), complementary DNA has been made from mRNA of several patients with breast tumor biopsies. The researchers would be looking for: a. a pattern of non-fluorescence that indicates actively transcribed genes from cells at a given time b. some particular genes that are expressed from mRNA to cDNA to rRNA c. a pattern shared among some or all of the samples that indicates gene expression differing from control groups d. all of the above e. none of the above A sequence base in the template strand of DNA is CACTGCGTCACC. The corresponding codon for the mRNA transcribed is: a. GUGACGCAGUGG b. GCGUCGCUGCGG c. CACTGCGTCACC d. TUGCATCUTAUG A specific area of a chromosome the sequence of nucleotides below is present where the chain opens to form a replication fork: CCTAGGTAACCGTTA. An RNA primer is formed at the underlined C of the template. What is the primer replication sequence on DNA? a. TAAGGCAAT b. GTTCCATTA c. UTTUCATTU d. ATTGGCAAT e. AUUGGCAAU A possible sequence of nucleotides in the template strand of DNA that would code for the polypeptide sequence leu-pro-lys-ala would be: (From the Table) a. UUA-CCU-AAG-GCU b. AGA-AAG-CCA-UUG c. UAC-CCC-AAA-GUU d. CUG-CUC-CCG-UGC e. CUT-DOG-CAT-TAG

Explanation / Answer

Answer:

69) Question is not visible.

70) A microarray assay (DNA chip), complementary DNA has been made from mRNA of several patients with breast tumor biopsies. The researcher would be looking for:

Answer: c) a pattern shared among some or all of the samples that indicates gene expression differing from control groups.

71) A sequence base in the template strand of DNA is CACTGCGTCACC. The corresponding codon for the mRNA transcribed is:

Answer: a) GUGACGCAGUGG

72) At a specific area of a chromosome the sequence of nucleotides below is present where the chain opens to form a replication fork.CCTAGGTAACCGTTA. An RNA primer is formed at the underlined T of the template. What is the primer replication sequence on DNA?

Answer: There is a typo in question. There is no underline C, Also, all the options seems wrong, the correct primer replication sequence on DNA would be TAACCGTTU

(Primer mRNA sequence will be AUUGGCAAU and replication DNA sequence will be TAACCGTTA)

73) A possible sequence of nucleotides in the template strand of DNA that would code for the polypeptide sequence leu-pro-lys-ala would be:

Answer: All the options seems wrong, as the template DNA would not contain Uracil.

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