When the human genome was first sequenced, scientists expected to find around 10

Question

When the human genome was first sequenced, scientists expected to find around 100 000 genes (which corresponded to the estimated number of different proteins in a human), Upon its completion and analysis of reading frames, they discovered the number was more likely 25 000. What is the most likely explanation for this? Imagine that a molecular biologist cuts out the amino acid sequence for eukaryotic gene and places it in a prokaryotic chromosome, just downstream of a prokaryotic promoter. What would be the most likely result, when RNA polymerase binds the promoter? _______ and _______ did an experiment with phases to show that DNA is the genetic element. What elements did they use to differentiate DNA from protein?______ Why did they not use N? (3) Two scientists called_____ and____ showed experimentally that DNA replicates in a______ manner. (2) Conserved sequences upstream of a gene where RNA polymerase binds in prokaryotes to start transcription in a DNA is called_______(1)

Explanation / Answer

5.It is most likely that human genome contains junk DNA(repetitive nucleotide sequences)that does not code for any protein.

6.RNA polymerase would transcribe the gene that is inserted.

7.Hershey and Chase .The elements they used were radioactive isotope of sulfur i.e 35S and radioactive isotope of phosphorus i.e 32 P.They did not use N because both protein and DNA contain nitrogen in their composition and it would become difficult to differentiate whether radioactive DNA or protein was injected into the host via phages.

8.Two scientists called Meselson and Stahl showed experimentally that DNA replicates in a semi conservative manner.

9.Pribnow box( present in promoter site).

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