What is the relationship between absorbance and concentration defined by the Bee

Question

What is the relationship between absorbance and concentration defined by the Beer-Lambert law?

absorbance is exponentially dependent on concentration

absorbance is directly proportional to concentration

absorbance is proportional to the concentration squared

absorbance is not proportional to concentration

absorbance is inversely proportional to concentration

2 points   

QUESTION 2

What are the limitations on the Beer-Lambert law?

the law is valid at absorbance values less than 2

the law is valid at any concentration

the law is valid at absorbance values greater than 2

the law is valid at absorbance values less than 0

1 points   

QUESTION 3

The max of Blue #1 is 628 nm. What wavelength will be analyzed on the MicroLab equipment?

The max of Yellow #5 is 427 nm. What wavelength will be analyzed on the MicroLab equipment?

(Both answers should have 3 significant figures and units of nm)

2 points   

QUESTION 4

A particular fabric dye absorbs light wavelengths around 580 nm. Use the visible spectrum below to determine the color of light absorbed by the dye.  

indigo

red

blue

yellow

violet

1 points   

QUESTION 5

The human eye interprets the remaining wavelengths as color. What color is perceived by our eyes for the fabric dye that absorbs around 580 nm?

red

blue

green

purple

yellow

1 points   

QUESTION 6

A serial dilution series is prepared for the fabric dye and analyzed using MicroLab equipment. Use Excel or a similar program to create a Beer-Lambert Law standard curve from the data given. Report the slope of the line to 3 significant figures (no units). Note: it may be necessary to increase the displayed decimal places in the equation of the line to obtain an accurate answer.

Concentration (mol/L)

Absorbance

0.040

1.71

0.025

1.05

0.010

0.39

0.004

0.17

2 points   

QUESTION 7

Serial dilution is an essential chemistry skill. Use the description given to calculate the concentration of each new solution. Report all answers to three significant figures, with units of M.

The concentration of the initial stock solution is 3.00 M. The first dilution is made by measuring 10.00 mL with a volumetric pipet and diluting in a 100 mL volumetric flask. What is the concentration of the first solution?

The second solution is made by measuring 25.00 mL of the first solution and diluting in a 100 mL volumetric flask. What is the concentration of the second solution?

The third solution is made by measuring 50.00 mL of the second solution and diluting in a 250 mL volumetric flask. What is the concentration of the third solution?

The fourth solution is made by measuring 1.00 mL of the third solution and diluting in a 500 mL volumetric flask. What is the concentration of the fourth solution? (Count the zeros carefully when entering an answer!)

8 points   

QUESTION 8

Stu Dent needs to make a 0.750 M solution from the 3.00 M stock solution. Only a 100 mL volumetric flask is available. Volumetric pipets of all different sizes are available. Determine the best volumetric pipet size to make the desired concentration.

1 mL

3 mL

5 mL

8 mL

10 mL

15 mL

25 mL

50 mL

1 points   

QUESTION 9

Kemmi Major needs to make an approximately 1.00 M solution from the 3.00 M stock solution. Only a 100 mL volumetric flask is available. Volumetric pipets of all different sizes are available. Kemmi realizes one pipet is not sufficient. She also knows each individual measurement made with a pipet creates a little more error. Help her choose the volumetric pipet sizes that give the fewest possible measurements to make the desired concentration.

1 mL

3 mL

5 mL

8 mL

10 mL

15 mL

25 mL

50 mL

1 points   

QUESTION 10

What is the actual final concentration of Kemmi’s solution (made in the previous question)? Answer with two significant figures and units of M.

absorbance is exponentially dependent on concentration

absorbance is directly proportional to concentration

absorbance is proportional to the concentration squared

absorbance is not proportional to concentration

absorbance is inversely proportional to concentration

Explanation / Answer

Question 1: Absorbance is directly proportional to concentration

Question 2: The law is valid at any concentration

Question 4: Yellow

Question 5: Blue

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