A) What role does the surfactant play in ion-pair chromatography? The charged, h

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A) What role does the surfactant play in ion-pair chromatography? The charged, hydrophilic end of the surfactant is attracted to the wall of the column. The hydrophobic end of the surfactant attracts the ions as they are introduced into the column Due to the highly controlled, temperature sensitive equilibrium between the hydrophobic end of the surfactant and the ions (cation or anion), the ions can be eluted from the column by increasing or decreasing the temperature. The surfactant is added to form micelles (ion pairs) around the analytes of interest, preventing nonspecific adsorption to the separator column as they are eluted. The surfactant then releases the ions in the suppressor column, which allows them to be detected O The surfactant is introduced into the column in the mobile phase and binds to the stationary O phase with its nonpolar, hydrophobic end. The charged end of the surfactant then acts as arn ion exchanger, attracting ions in the sample of opposite charge. The surfactant is added to the mobile phase at a value equal to its critical micelle concentration. Micelles are formed around the ions of interest in the sample and are passed through the column and separated based on micelle-ion pair size. The larger micelle-ion pair species will elute from the column first

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a   4th option

b   3rd option

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