The following figure illustrates the genomic DNA structure of the dumpy gene in

Question

The following figure illustrates the genomic DNA structure of the dumpy gene in Drosophila melanogaster. The promoter region is about 500 bp long, exon 1,600 bp, intron 1,600 bp, and exon 2,300 bp. Primers a, b, c, d, e, f, and g are each about 20 nucleotides long.

Peter identified five mutants of dumpy gene as listed, based on the given information predict the corresponding mRNA and protein length derived from the wild type and from each of the mutated alleles.

primer a primer b primer c primer d primer e primer f primer g primer h promoter Intron 1 +1 +600 /601 +1200/1201 +1500 Initiation codon ATG (A is at n.t. position +201) Stop codon TAG (G is at n.t. position +1403)

Explanation / Answer

Exons are coding regions. They are transcribed into mRNA which are then subsequently translated to proteins. Triplet codon of mRNA codes for one amino acid.

Here, exon1 is of 1600 bp in length and exon2 is of 2300 bp in length. Therefore, the total length of coding sequence is 3900 bp which contains 1300 triplet codons.

dp1 - missense mutation in codon 12

dp2- nonsense mutation in codon 12

dp3 - mutation in promoter that inactivates transcription

dp4 - mutation in nt position +900 did not change splicing pattern

dp5 - mutation in nt position +602 changing the splicing pattern

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