4. The following sequence is found on the template strand of DNA. First, determi

Question

4. The following sequence is found on the template strand of DNA. First, determine the amino acids of the polypeptide encoded by this sequence by using the genetic code. Then give the altered amino acid sequence of the polypeptide that will be found in each of the following mutations: Sequence of DNA template: 3'–TAC TGG CCG TTA GTT GAT ATA ACT–5' Nucleotide number                                                                                                                                                                                      1                                                           24

a. A transition at nucleotide 11                                

b. A transition at nucleotide 13

c. A one nucleotide deletion at nucleotide 7                                                            

Explanation / Answer

Template strand is,

3' - TAC TGG CCG TTA GTT GAT ATA ACT - 5'

The corresponding mRNA is,

UAC UGG CCG UUA GUU GAU AUA ACU

The corresponding amino acid sequences in protein are,

Tyr-Trp-Pro-Leu-Val-Asp-Iso-Thr

a) Transition at nucleotide 11

DNA 3'- TAC TGG CCG TCA GTT GAT ATA ACT -5'

mRNA UAC UGG CCG UCA GUU GAU AUA ACU

Protein: Tyr-Trp-Pro-Ser-Val-Asp-Iso-Thr

b) transition at nucleotide 13

DNA 3'- TAC TGG CCG TTA ATT GAT ATA ACT - 5'

mRNA UAC UGG CCG UUA AUU GAU AUA ACU

Protein: Tyr-Trp-Pro-Leu-Iso-Asp-Iso-Thr

c) One nucleotide deletion at nucleotide 7,

DNA 3'- TAC TGG CGT TAG TTG ATA TAA CT -5'

mRNA UAC UGG CGU UAG UUG AUA UAA CU

Protein: Tyr-Trp-Arg-stop codon

Related Questions

Navigate

• Browse (All)
• Browse 4
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.