How much agarose would you need to weight to make 100 ml of a 12% agarose gd? Ho

Question

How much agarose would you need to weight to make 100 ml of a 12% agarose gd? How much 40XTAE buffer would you need to add to make 1 liter of 1X buffer? How much water would you need to add? I have 5M Tris buffer and need to make 500ml of 500mM Tris. How much SM Tris do need? One of the steps in genomic DNA isolation requires 70% ethanol to precipitate the DNA. How much 100% ethanol do I need to make 50ml of 70% ethanol? How much water do I need to add? I have a 50 mg/ml ampicillin stock and need to make 500ml of media containing 50ug/mL ampicillin. How many microliters of 50mg/ml ampicillin do lad?

Explanation / Answer

1 . 100 % gel would be 100 g agarose in 100 ml TAE buffer.

So 1.2% gel would be 1.2 g agarose in 100 ml TAE buffer.

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