Assume you have four different libraries: a genomic library made from DNA isolat

Question

Assume you have four different libraries: a genomic library made from DNA isolated from human brain tissue, a genomic library made from DNA isolated from human muscle tissue, a human brain cDNA library and a human muscle cDNA library. a. Which library (or libraries) would have the greatest diversity of sequences? b. Would the sequences contained in each library be expected to overlap completely, partially, or not at all with the sequences present in another library? c. In which library (libraries) would you find the following (if you don't know the function of the gene listed, please search for a description on-line): -The exon of an actin gene -An intron of an actin gene -A promoter of an actin gene -An exon of the neuroD gene

Explanation / Answer

A) Either brain genomic library or muscle genomic library, C- DNA libraries will have the lesser sequences than the genomic libraries.

B) Both genomic libraries overlap completely and the both c DNA libraries would overlap partially but there will be no libraries that doesn't overlap at all because it is subset of genomic library only.

Exon of an actin gene would be found in both genomic libraries and cdna library of muscle.

Intron of an actin gene would be found in both genomic libraries as introns will cut out from the both c DNA libraries.

Promoter of an actin gene would be found in both genomic libraries because promoter sequences are not present in cdna library as it only contains the exons.

An Exon of the neuroD would be found in both genomic libraries and cdna library of brain.

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.