Two isotopic compounds are produced in the synthesis of a new therapeutic drug w
ID: 1019522 • Letter: T
Question
Two isotopic compounds are produced in the synthesis of a new therapeutic drug which must be separated by repeated chromatography cycles. In each cycle, the solution containing both compounds passes through a chromatography column of length L = 20.0 cm containing N theoretical plates. The compounds have an adjusted relative retention, , of 1.011 and retention factors of 5.01 and 5.07, respectively. Calculate the number of theoretical plates, N, in the column of length L. The observed resolution is 1.62 after 13 cycles. Calculate the height of an individual plate in micrometers.Estimate the resolution after 3 cycles.
Explanation / Answer
number of plates=5.545(tr/wh)2;where tr: retention time, and Wh: peak
Resolution after 13 cycles are given and from that we have to calculate the peak width by tangent line method or half peak method
resolution=1.18((tr2-tr1)/(wh1+wh2))
From the above equationwe will be getting the peak width and usingthat we can calculate number of plates.
next step is
Height of the plate=(Length of the column/number of the plates)*1000mm/M
At last, find the resolution for 1 cycle and the find the resolution after 3 cycles is obtained by above mentioned equation
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