Chem 407 (Biochemistry I) Exam II (3/19/2018) Name: 1. (22 pts) Answer each of t

Question

Chem 407 (Biochemistry I) Exam II (3/19/2018) Name: 1. (22 pts) Answer each of the following questions by choosing the right number belovw (1) RFLPs (5) intron (2) semiconservative (6) sickle-cell anemia (3) hyperchromicity (7)genetic code (4) PCR (8) Bohr effect (10) Southern blotting (11) restriction enzymes (a) The symmetry of the subunits are maintained (b) A variation in the DNA sequence of a genome that can be detected by breaking the DNA into pieces and analyzing the size of the resulting fragments by gel electrophoresis. (c) used by certain bacteria to block phage infections (d) The nucleotide sequence within a gene that is removed by during the RNA processing. (e) DNA replication produces two copies that each contained one of the original strands and one entirely new strand. 0 mutations in genes encoding hemoglobin (g) If the sequence of at least part of a DNA segment is known, the segment can be amplified by this method (h) CO2 and H' on the oxygen binding curve (i) Separates and identifies a specific DNA fragment. G) The relationship between the sequence of bases in DNA (or RNA transcripts) and the sequence of amino acids in proteins. (k) Increase of UV absorption upon denaturation of DNA duplex. 2. (6 pts, No partial credit) An autoradiogram of a sequencing gel containing four lanes of DNA fragments is shown below. What is the sequence of the DNA fragment? (5' to 3' direction) G A

Explanation / Answer

a) concerted model also called as symmetry model

b) restriction enzymes which cuts dna into fragments and these fragments are separated by gel electrophoresis as it separates dna fragments on the basis of charge and mass

c) genetic code

d) intron is removed by splicing because it is non coding part

e) semiconservative

f) sickel cell anaemia which is caused due to point mutation which results in substitution of glutamic acid by valine

g) PCR ( polymerase chain reaction ) which include three steps : denatueation ; annealing and extension

h) Bohr's effect is shifting of hemoglobin oxygen dissociation curve to the right.

i) RFLP ( restriction fragment length polymorphism)

j) southern blotting which is a hybridisation technizque between dna and rna

k) hyperchromacity

Related Questions

Navigate

• Browse (All)
• Browse C
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.