The figure shows the separation of two enantiomers on a chiral stationary phase.

Question

The figure shows the separation of two enantiomers on a chiral stationary phase. Separation of enantiomers of Ritalin by HPLC with a chiral stationary phase. [Data from R. Bakhtiar, L. Ramos, and F. L. S. Tse, "Quantification of Methylphenidate in Plasma Using Chiral Liquid-Chromatography/Tandem Mass Spectrometry: Application to Toxicokinetic Studies, "Anal. Chim. Acta 2002, 469, 261.] From t_r and w_1/2, find N for each peak. From t_r and w_1/2, find the resolution. Given t_m = 1.62 min, use Equation 23-33 with the average N to predict the resolution.

Explanation / Answer

From the chromatographic chart

(a) N for L enantiomer

= 5.54(Tr/W1/2)^2

= 5.54(4.70/0.28)^2

= 1560.98

N for D enantiomer

= 5.54(5.37/0.35)^2

= 1304.15

(b) resolution

= (tr2 - tr1)/(Wb1/2)

= (5.37 - 4.70)/(0.35 + 0.28)

= 1.0635

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