12. You have identified a series of mutations in bacteria that cause them to be

Question

12. You have identified a series of mutations in bacteria that cause them to be deficient in glutamine production. These mutations are found in genes A, B, and C, which produce proteins A, B, and C. Based on your growth of these mutants orn minimal media supplemented with either ?-Ketoglutarate, Glutamate, or Glutamine (see table below), what is the order of enzymes in the biochemical synthesis pathway of glutamine? no supplement a-Ketoglutarate Glutamate Glutamine wild-type mutant A mutant B mutant C a. C->A->B d. B-A>C e. B->C->A 13. A tRNA anticodon sequence of 3'-CUG-5' can pair with which mRNA sequence(s)? b. 5-GAU-3 .5-GGU-3 d. a and b only e. a and c only a. 5'-GAC-3 14. Damaged mRNAs that end without a stop codon being present are recognized during translation and the translation of the damaged mRNA is completed (and the protein product targeted for degradation) using a tmRNA through a process called: a. splicing b. mRNA editing c. mRNA surveillance d. transcription 15. A positive repressible operon is regulated by a control protein that is initially produced as an: a. inactive activator b. active activator c. active repressor d. inactive repressor

Explanation / Answer

Answer 12) The order of enzymes in the biochemical synthesis pathway of glutamine is C--->A------>B.

Answer 13) 3' CUG 5' pairs with 5' GAU 3'.

Answer 14) transcription.

Answer 15) active activator.

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