12. You have identified a series of mutations in bacteria that cause them to be

Question

12. You have identified a series of mutations in bacteria that cause them to be deficient in glutamine production. These mutations are found in genes A, B, and C, which produce proteins A, B, and C. Based on your growth of these mutants orn minimal media supplemented with either ?-Ketoglutarate, Glutamate, or Glutamine (see table below), what is the order of enzymes in the biochemical synthesis pathway of glutamine? no supplement a-Ketoglutarate Glutamate Glutamine wild-type mutant A mutant B mutant C a. C->A->B d. B-A>C e. B->C->A 13. A tRNA anticodon sequence of 3'-CUG-5' can pair with which mRNA sequence(s)? b. 5-GAU-3 .5-GGU-3 d. a and b only e. a and c only a. 5'-GAC-3 14. Damaged mRNAs that end without a stop codon being present are recognized during translation and the translation of the damaged mRNA is completed (and the protein product targeted for degradation) using a tmRNA through a process called: a. splicing b. mRNA editing c. mRNA surveillance d. transcription 15. A positive repressible operon is regulated by a control protein that is initially produced as an: a. inactive activator b. active activator c. active repressor d. inactive repressor

Explanation / Answer

Ans.12

The order of enzymes in the biochemical pathway is C -> A -> B.

As mutant C is able to survive in the presence of alpha-ketoglutarate, glutamine and glutamate, the mutation must be affecting the pathway downstream of alpha - ketoglutarate. similarly mutant B can survive only in the presence of glutamine which means the mutation has affected the enzyme immediately downstream of the glutamate.

Ans.13

d. a and b only.

As G at wobble position can pair with both C and U.

Ans.14

c. mRNA surveillance

Ans.15

d. inactive repressor

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