12. You have a restriction enzyme that cuts once in the middle of a 12KB circula

Question

12. You have a restriction enzyme that cuts once in the middle of a 12KB circular plasmid. What size bands do you expect to see if you run the product(s) of your restriction digest on a gel? Question 12 options:

A) Two bands very close together on the gel

B) One very thick/bright band at 6KB

C) One band at 12KB

D) No bands

The purpose of a mini prep is to:

Question 13 options:

amplify the DNA from bacteria cells

amplify the RNA from bacteria cells

isolate and purify a DNA plasmid from bacteria cells

Insert a DNA plasmid into a bacterial cell

A)

amplify the DNA from bacteria cells

B)

amplify the RNA from bacteria cells

C)

isolate and purify a DNA plasmid from bacteria cells

D)

Insert a DNA plasmid into a bacterial cell

Explanation / Answer

12. B) One very thick/bright band at 6KB

If the cut is exactly in the middle then a thick band is seen which indicates the presence of more than one fragment of the same size.

13. isolate and purify a DNA plasmid from bacteria cells

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