Techniques in cell biology 5. You are instructed to make 50 ml of the folowing c

Question

Techniques in cell biology 5. You are instructed to make 50 ml of the folowing culture medium 1.4% (NHalsa Yeast minimal medium (2x): 4% D-glucose, 0.3% yeast nitrogen base, You are given each in powder form. How much of each ingredient do you need to weigh out? 6. the normal level found in living Physiological saline is 0.9% (w/v) for mammals.(Physiological means systems; saline means salt, usually NaCi, or the combination of salts that supports living systerns which includes small amounts of KCL, MgCla, CaC In preparing physiological saline for an experiment, you are asked to use NaCi (Mw- 58.44) a. How much NaCIl would you weigh out to make 50 ml of solution? b. What is the molarity of your solution? 7. You are given a 3 mg/mi stock solution of bromphenol blue. You are directed to make three serial 1:10 dilutions with water a. In order to make a total of 5 ml of the first 1:10 dilution, how much of the bromphenol blue solution would you use? How much water? b. What is the overall dilution factor for the final diluted sample after the three serial dilutions? c. What is the concentration in g/ml of the final diluted sample? d. What would you call a dilution that is done by mixing 0.2 ml of the original sample with 9.8 ml of H:0? 144

Explanation / Answer

Question 5-

First consider the amount of D-glucose that is 4%. This suggests that we need 4 grams of D-glucose in 100 ml of water.

For 50 ml of solution the required grams of D glucose = 4 x 50ml/100ml

= 2 grams

Now 0.3% yeast nitrogen base, which means 0.3 grams in 100 ml water. For 50 ml it must be 0.15 grams.

For 1.4% (NH4)2 SO4 we need 1.4 grams in 100 ml water. For 50 ml of the solution we just need 0.7 grams.

Now add all three ingredients in half of the required amount of solution, which is 25 ml. we will mix them in distilled water. After adding little level of the water will increase due to solutes. After mixing, complete the volume of solution up to 50 ml by adding more water.

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