WC 1 isgona that has been implicated in cancar. Aternatve solong of WC 1 reits n
ID: 140626 • Letter: W
Question
WC 1 isgona that has been implicated in cancar. Aternatve solong of WC 1 reits n teproductn of to protan s torm the wc gane, -KTS and-KTS The fgure bolow shows a DNA binding aftinity assay In a DNA binding assay an ecic eld is applied to a gel loaded with ONA and proten Smaller molecules wil traval urther through the gel than larger moleoues +Ph is a short sequence ot DNAa WC1 proteins typicality bind to-PS was loaded into ane 1 of the gal by tsaitf (no protain Lane2 was loaded with only Pa KTS pratars (na DNALane 3 wasiaased we, ban .P5 -KTS Lane 4 was oaded win bath *PS AND KTS Direction of Migration +P5 What does this gure tell us about the binding of WC1 proteins-KTS andKTS)to -PS DNATExplanation / Answer
Generally in DNA binding affinity of protein in gel electrophoresis the end labeled DNA retard the motility of the fragment and makes discrete bands on gel electrophoresis.
1st lane is p5 loaded which shows only a discrete bands
In lane 2 only proteins are added no DNA it also shows a single band
3. P5 and -KTS added but it also showed single band at the end means -KTS does not bind to P5
In lane 4 p5 and +KTS are added showed 2 discrete bands which means P5 binds to + KTS protein because of which motility is slowed down and 2 bands are formed
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