E) the original electrons are used to drive the light reactions Use the standard
ID: 141435 • Letter: E
Question
E) the original electrons are used to drive the light reactions Use the standard curve provided to answer questions #14-15. y 0.02x-0 R2 = 0.9977 14. You are tasked with determining the 0.25 solution whose absorbance is 0.15. The0.20 0.15 0.10 0.05 concentration of an unknown protein8 approximate protein concentration is C) 7.5 g/ml ) 11 g/ml E) Cannot be determined with the information provided 0.00 0 2 4 6 810 12 14 16 15. Your lab director requires you to Protein Conc. ug/ml dilute an unknown protein 10 times in order for you to measure its concentration. The diluted sample gives an absorbance of 0.08. Estimate the concentration in the ORIGINAL (undiluted) protein sample. A) 0.8 mg/ml D)/4 g/ml E) 6 mg/ml 40 ug/ml
Explanation / Answer
14.
The equation of a straight line is y= mx + c
In this equation, y refers to the y coordiante, x refers to the x coordinate, m refers to the slope of the line with respect to the x-axis and c denotes the y intercept.
the equation for the line on the graph has been given to us in the question : y = 0.02x - 0. .......... (1)
we want to find out what is the Protein concentration for an absorbance of 0.15.
therefore, the y coordinate is 0.15.
simply putting this value in the (1) equation,
y = 0.02x - 0
0.15 = 0.02 x - 0
0.15 = 0.02 x
0.15/0.02 = x
7.5 µg/ml = x
The unit is µg/ml and not µg/µl because the y axis has the unit of µg/ml.
15.
let the concentration of the unknown protein be a.
if you dilute that protein 10 times, the concentration of the diluted solution will be ten times lesser than that of the original solution = a/10.
the absorbance of the diluted solution (a/10) is 0.08.
Using the equation of the line, y=0.02x -0
we get, 0.08 = 0.02x - 0
0.08 = 0.02x
x = 0.08/0.02
x=4 µg/ml
4 µg/ml denotes the concentration of the diluted solution i.e. a/10.
That means, to get the conccentration of the original solution (a), we will have to multiply 4 µg/ml by 10.
4 µg/ml x 10 = 40 µg/ml.
This is the concentration of the original solution.
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