Ciare Halee Transeription and Translation Worksheet Suppose the original DNA mes

Question

Ciare Halee Transeription and Translation Worksheet Suppose the original DNA message is this S' ATG GAT GCT CTT GAA TTT AAA TAA 3 3' TAC CTA CGA GAA CTT AAA TTT ATT S The corresponding mRNA would then be: The tRNAs that (individually) matched the mRNA would then be: And the protein that would be formed would be: Now, suppose there are alterations in the DNA. The codons IN DIFFERENT FONT are altered. Work through each of the following five examples. Then go back and explain the type of mutation that occurred in each situation. 1. 5 ATG GAT GCT CTT GAA TTT AAA TAA 3 3 TAC CTA CGA GAT CTT AAA TTT ATT S The corresponding mRNA would then be And the protein that would be formed would be: 2. 5' ATG GAT GCT CTT GAA TTT AAA TAA 3' 3' TAC CTC CGA GAA CTT AAA TTT ATT 5' The corresponding mRNA would then be: And the protein that would be formed would be:

Explanation / Answer

Answere : Transcription and translation worksheet :

Answere: For following question "Suppose the origin messege DNA messege is this "....

Part I : Corresponding mRNA sequence for above DNA sequence is : (As mRNA synthesized in 5'-3' direction)

5' AUG GAU GCU CUU GAA UUU AAA UAA 3'

Part II : The tRNA sequence (i.e. anticodon sequence) is complementary to mRNA sequence. So, tRNA sequence for above DNA sequence is :

3' UAC CUA CGA GAAC CUU AAA UUU AUU 5

Part III : The protein formed from above mRNA sequence is :

Met-Asp-Ala-Leu-Glu-Phe-Lys.

Question 1 :

Part I : The above mutation is transversion mutation in which a purine replaces a pyrimidine or vice versa (in this case Adenine is replaced by Thymine) The mRNA sequence for above mutation in DNA will be : 5' AUG GAU GCU CUA GAA UUU AAA UAA 3'

Part II : The protein formed by above mRNA sequence would be : Met-Asp-Ala-Leu-Glu-Phe-Lys.

Question 2 : It is also a type of transvesion mutation. The mRNA sequence for above mutation in DNA will be :

5' AUG GAG GCU CUU GAA UUU AAA UAA 3'

Part II : The protein formed by above mRNA sequence would be : Met-Glu-Ala-Leu-Glu-Phe-Lys.

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