please I want the show work for the whole question? 1. (8 points) During lab, yo
ID: 144211 • Letter: P
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please I want the show work for the whole question?
1. (8 points) During lab, you used a set of BSA standards with known concentration by diluting a 5 mg/ml stock solution of BSA. Calculate the final concentration of BSA in the problems below using the formula where C1 and V1 are the concentration and volume of the starting solution, and C2 and V2 are the concentration and volume of the final diluted solution. Include proper units and show your work for full credit. A. 300 .of 5 mg/ml BSA is added to 700 ul.TBS B. 50 ul of 1.5 mg/ml BSA is mixed with 450 ul H20 and 500 ul Bradford Reagent C. 10 .of 1 mg/ml BSA is mixed with 990 ulTBS. D. 10 ulof 0.1 mg/ml BSA is mixed with 990 ul TBS.Explanation / Answer
C1=Concentratin of the starting solution.
V1=Volume of the starting solution.
C2= Concentration of the final diluted solution
V2=Volume of the final diluted solution
a. V1=300 ul = 0.3 ml(1ml =1000ul),C1 = 5mg/ml
V2 = 700 ul =0.7ml ,C2 = to be find out
C1 X V1 = C2 X V2
5 X 0.3 = C2 X 0.7
C2 = 5 X 0.3 / 0.7 = 2.14mg/ml
b. V1= 50Ul = 0.05ml, C1= 1.5mg/ml
v2 = 450+ 500 ul =950ul =0.95ml,c2 =to be find out
c1v1 = c2v2
1.5 x 0.05 = c2 x0.95
c2 = 1.5 x 0.05/ 0.95 = 0.075 / 0.95 = 0.078 mg/ml
c. v1 = 10ul = 0.01ml, c1 = 1mg/ml
v2 = 990ul = 0.99ml, c2= to be find out
c1v1 =c2v2
1 x 0.01 = c2 x 990
c2= 1x 0.01 / 990 = 0.0001 mg/ml
d. V1= 10UL = 0.01, C1 = 0.1mg/ml
v2 = 990ul = 0.99, c2 = to be find out
c1v1 = c2v2
0.1 x 0.01 = c2 x 0.99
c2 = 0.1 x 0.01 / 0.99
=0.001 / 0.99 = 0.001 mg/ml
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