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Review View Help Tell me what you want to do AaBbCcDd AabceDd AaBbC AaBbcel AaB AaBbCo Aa 1 Normal 1 No Spac... .Heading 1 Heading2 TitleSubtitle Su Aa Paragraph Samples setup Styles 6. Set up two 1.5 mL microfuge tubes for each dye concentration labeled with the concentrations. Transfer 200 jul of each dye from last week into its respective tube. To a seventh pair of tubes, add an equal volume of yeast medium alone, this is a 0% dye sample, and will be your "background" control that allows you to determine absorbance due to the ight scattering by yeast cell alone. Additionally, label one set of tubes "Control" and label the other tubes+ Azide" Experimentation: Metabolic inhibition of ATP producti 7. When the yeast cells have completely re-hydrated, measure out 1.8 mL of well-mixed yeast suspension (from step 4) into each of two new 15 mL centrifuge tubes. 8 WEARING GLOVES AND USING EXTREME CAUTION, add 200 mL of a 10% (w/v) Sodium Azide"in YGM solution to one of the 15 mL tubes with yeast suspension. Close the tube and vortex briefly (-10 seconds) to mix. 9. Add 200 pul of YGM to the other 15 mL tube of yeast suspension for your Control. Close the tube and vortex briefly (2-3 seconds) to mix. 10. Let both tubes incubate at room temperature for 30 min, vortexing every -5 minutes. Q6. In these samples you have the actual conditions under which metabolic inhibition of ATP production by sodium azide takes place. Record these conditions What is the % concentration of azide in this sample during metabolic inhibition? Lab 1: How do yeast regulate the fnow of molecules in and out of the cell? What is the % concentration of yeast in this sample during metabolic inhibition?Explanation / Answer
As per the data provided in the image :
Yeast suspension = 1.8 ml (This will be taken as amount of yeast as the mass of yeast has not been given in the image)
Sodium azide in YGM added = 200 microlitre = 0.2 ml
Total volume = 2.0 ml
a) Now, % of azide in sample can be calculated as:
Total volume in sample tube = 2.0 ml
Sodium azide = 10% w/v in YGM. It means 10 g of sodium azide is present in 100 ml of YGM
Sodium azide solution added to sample = 0.2 ml
Mass of sodium azide can be calculated as :
100 ml of YGM solution contains 10 g of sodium azide
0.2 ml of YGM solution contains (10 g/100 ml) x 0.2 ml = 0.02 g
Concentration of sodium azide in sample tube = mass of sodium azide in sample tube/ total volume in sample tube
Concentration of sodium azide in sample tube = 0.02 g/ 2.0 ml = 0.01g/ml
In percent it is , 0.01 x 100 = 1% (w/v)
b) To calculate % concentration of yeast in sample
Volume of yeast suspension = 1.8 ml
Total volume of solution = 2.0 ml
Concentration of yeast = Volume of yeast suspension / Total volume of solution
Concentration of yeast = 1.8/2.0 = 0.9
In percent it is 0.9 x 100 = 90% (v/v)
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