1. What is serum? How is it isolated? What nutrients does it provide to cells? 2

Question

1. What is serum? How is it isolated? What nutrients does it provide to cells? 2. What are some disadvantages of supplementing media with serum? 3. How much does serum cost? Compare the price of calf serum (CS), fetal bovine serum (HS), and iron supplemented calf serum (ISCS) Complete the cost table for the amount of serum required to prepare 1L of medium containing one of the following 4. Serum 10% FBS 5% FBS 10% CS 5%CS 5% CS: 5% FBS 10% HS 596 HS 5% HS, 5% FBS Cost 10% ISCS 5% 1SCS 5. From a cost only perspective, which serum would you select? Suggested vendors: Therme Fisher Scientific. Sigma. VWR

Explanation / Answer

1- serum is plasma minus fibronogen and clotting factor 2,5, 8 because these factors get consumed during clotting , if we allowed blood to clot in test tube , then clot retract and gives serum. Serum has high serotonin content beacuse of breakdown of platelet during clotting .

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