15. You are performing an experimen to study the differentialC expression of PEC

Question

15. You are performing an experimen to study the differentialC expression of PECAM-1 mRNA in different tissues using northern blot analysis. You have obtained following gel images. Based on the results, is it sufficient to conclude that PECAM- expression is higher in kidney than in brain or heart (mRNA) PCAM-1 tissue? (3pt) Ifyes, bricfly explain why it is suficient to support the conclusion Ifno, briefly explain why it is not sufficient to support the conclusion and what additional measurement should be done to support the conclusion.

Explanation / Answer

Answer: No

Explanation: Northern Blotting technique is used to quantify the mRNA levels present in a tissue. It can be used as a quantitative method. For the quantitative purpose, we need a loading control. We can compare the levels of the test mRNA with the internal control such as rRNA and describe the result.

In the given case, there is no internal/loading control. Without the internal control, we cannot say that a gene is more abundant in one sample than the other.

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