Cyanide, oligomycin, and 2, 4-dinitrophenol are all inhibitors of oxidative phos
ID: 162416 • Letter: C
Question
Cyanide, oligomycin, and 2, 4-dinitrophenol are all inhibitors of oxidative phosphorylation in mitochondria. Answer the following questions about these potent inhibitors. Explain why adding cyanide to an active in vitro suspension of mitochondria blocks ATP synthesis. What happens to the rate of ATP synthesis when 2, 4-dinitrophenol is added to a mitochondrial suspension after it was treated with cyanide? Explain. Explain why the rate of oxygen consumption decreases in an in vitro suspension of mitochondria when oligomycin is added. What happens to the rate of oxygen consumption in this oligomycin-inhibited system after adding 2, 4-dinitrophenol? Explain.Explanation / Answer
A. Cyanide is a chemical compound that contains monovalent cyanide group. Cyanide is toxic because it binds with cytochrome C oxidase I.e. the 4th complex in ETC. It attaches to the iron and inhibits the normal process of the complex system. It binds tightly so that it cannot bind to any electron to oxygen in the transport system and effecting the further transport of electron through the chain causing a halt in the Mitochondrial ATP production.
2. On treating with 2,4 DNP after cyanide treatment the process will start again that is the production of ATP will be accessed.
3. Oxygen consumption rate decreases after adding oligomycin because ATP synthesis is inhibited which is coupled to the Electron transport chain.
4. The oxygen rate will start normally after being treated with 2,4 DNP
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