Compare the techniques used to prepare a lawn culture and streak culture. Compar

Question

Compare the techniques used to prepare a lawn culture and streak culture. Compare the purpose for each type of culture. What is the significance of the clear zones around some of the antiseptic/disinfectant disks used in the Controlling the Growth of Bacteria exercise? Based on what you learned in the Controlling the Growth of Bacteria, what would you suggest as an effective way to clean a portable home which is typically a good environment for the growth of biofilms? Since the 1980's infectious disease-related deaths in the us are increasing significantly speculate on possible factors that may be contributing to this increase? In 1929, Alexander Fleming discovered the first antibiotic when he noticed that colonies of certain bacteria growing on a culture plates appeared to die the plates became contaminated with the fungus Fleming concluded that substance secreted from the fungus was causing the bacteria to breakdown, and he called this substance in its natural environment, what would be the adaptive advantage of a fungus producing and secreting a bacterial inhibitor?

Explanation / Answer

1. Streak Culture:

In microbiology, streaking is a technique to isolate a pure strain from a single species of microorganisms, often bacteria. Samples can be taken from resulting colonies and a microbiological culture can be grown on a new plate so that organism can be identified.

It is routinely used method to isolate bacteria.

Method:

Lawn culture: It is also called the carpet culture. It provides a uniform growth of the bacterium. It is most useful for bacteriophage typing and antibiotic sensitivity testing. It is also used in the preparation of bacterial antigens and vaccies.

Method:

2. The zone around an antibiotic disk that has no growth is referred to as the zone of inhibition since this approximates the minimum antibiotic concentration sufficient to prevent growth of the test isolate. This zone is then measured in mm and compared to a standard interpretation chart used to categorize the isolate as susceptible, intermediately susceptible or resistant. MIC measurement cannot be determined from this qualitative test, which simply classifies the isolate as susceptible, intermediate or resistant.

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