What genetic engineering method could you use in order to make a protein that is

Question

What genetic engineering method could you use in order to make a protein that is normally constitutively held in the cytosol accumulate in the ER?

What genetic engineering method could you use in order to make a protein that is normally constitutively held in the cytosol accumulate in the ER?

1.add a hydrophobic sequence of amino acids to the start of the protein 2.add an amino acid sequence that would result in a mannose - 6 phosphate group added 3.mutate an amino acid that would normally be phosphorylated to a different amino acid that cannot be phosphorylated 4.none of these would cause the protein to accumulate in the ER 5.fuse a GFP tag to the protein

Explanation / Answer

2.add an amino acid sequence that would result in a mannose - 6 phosphate group added

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