2-14 Chemical Germicides: Disinfectants and Antiseptics (p. 135. first paragraph

Question

2-14 Chemical Germicides: Disinfectants and Antiseptics (p. 135. first paragraph under "Theory") the student will be able to... Explain the difference between disinfectants and antiseptics Explain the two approaches (laboratory methods vs. in-use methods) that are useful in evaluating the effectiveness of chemical agents and identify the purpose of each approach Compare and contrast two procedures (tube dilution procedure and fingertip antisepsis procedure) to evaluate the effectiveness of chemical agents Define the term minimum inhibitory concentration (MIC) and determine the MIC from experimental data Analyze data to determine which chemical agent is most effective in the tube dilution procedure and fingertip antisepsis procedure in Lab: a) Working as table teams, prepare dilutions of one of the following chemical agents and assess its ability to inhibit the growth of Staphylococcus aureus. Ethyl alcohol Beta dine R Lysol R Working as individuals, assess the effectiveness of the following chemical agents as an antiseptic agent for the fingertips, compared to water as control: Explain how disinfectants differ from antiseptics. State a specific purpose for the tube dilution procedure and a specific purpose for the fingertip antisepsis procedure. After incubation. assess each tube in the tube dilution series for growth (G) or no growth (NG)of s aureus. Record your results on the class database; and construct a table of the class results. After incubation, assess the amount of growth in each quadrant of your plate using a 0 to+3 scale. Construct a table of your results and the results of three other students. for the tube dilution procedure, use the class data table to determine the minimum inhibitory concentration (MIC) of each agent for S. aureus. Next, determine which chemical agent is most effective in inhibiting growth of S. aureus. Explain your rationale. for the fingertip antisepsis procedure, use your data table to determine which germicidal agent is the most effective in inhibiting bacterial growth. Explain your rationale.

Explanation / Answer

Antiseptics and disinfectants are both used to destroy or to block the increase of microorganisms, despite that, they fluctuate on the position they are used. An antiseptic is used on alive tissues and cells to demolish any types of infections which may be living on the tissue. Disinfectants are bound to destroy microorganisms which can pollute nonliving objects. Regular antiseptics consider mouthwash, and yeast infection cure creams.Disinfectants are ordinarily wicked in domesticated surfactant yields for kitchens, bathrooms, and alternative commonly gripped items where germs are found. Antiseptics and disinfectants both perform to kill microbes, but antiseptics must additionally cause compressed or no offensive to the tissues of the body, whereas, disinfectants must not be reliable for computation on living tissue.

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