Complete the sentences below using the words and phrases, to review the automate

Question

Complete the sentences below using the words and phrases, to review the automated process of Sanger sequencing (Not all words and phrases used). The first step is the generation of a complete series of ___ complementary to the portion of DNA temple being analyzed. These strands are mixed with ___ plus the four deoxynucleotide triphosphates (ATP, TTP, GTP, and CTP) Four labeled dideoxynucleotides are also included in the mixture: ddATP, ddTTP, ddGTP, and ddCTP. In automated Sanger sequencing, each of these dideoxynucleotides has been with a ___ that produces a unique color. When a ___ is incorporated into a DNA chain by chance, polymerization of that chain ___. After polymerization is completed, the DNA is denatured to release the templates. What remain is a collection of DNA: unlabeled template strands and ___ Fragments are separated on a high resolution polyacrylamide gel that allows fragments differing by ___ in length to be distinguished. The largest fragments migrate the ___ distance. A scanning laser causes the labeled dideoxynucleotides to fluoresce, and the ___ of each fragment is recorded. The color data is then translated into ___ by "base calling" software.

Explanation / Answer

1.the first step is the generation of the complete series of single stranded sub fragments complementary to the portion of DNA template being analysed

2. these strands are mixed with DNA polymerase plus the four deoxynucleotide phosphate

3. four labeled dideoxynucleotide are also included in the mixture:ddATP, ddTTP,ddGTP,ddCTP in automated sanger sequencing each of these dideoxynucleotide has been labeled with flourscent dye that produces unique colour.

4.when a dideoxynucleotide is incorporated into a DNA chain by chance, polymerization of that chain stops at that point

5. after polymerization is completed th DNA is denatured to release the templates. what remains is a collection of DNA:unlabeled template strnads and four nucleotides

6. fragments are seperated o a high resolution polyacrylamide gel that allows fragments differing by only a single nucleotide in lenght to be distinguished. The largest fragments the shortest distance.

7. A scanning laser causes the labeled dideoxynucleotides to flouresence, and the labeled synthesized fragment of each frgment is recorded.

8. the color data is then translated into sequence data by base calling

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