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Biology Question When performing a transformation reaction using pBlu, you decid

ID: 174063 • Letter: B

Question

Biology Question

When performing a transformation reaction using pBlu, you decide to also add a negative control in which no vector was added to your competent cells before heat shock. When plating onto LA Amp, you realize that the negative control plate has 230 colonies. What is NOT a possible reason for this unexpected growth? You forgot to add antibiotics to the plate. You contaminated you competent cells with. You accidentally added vector to the cells. You added too much antibiotic to the plate.

Explanation / Answer

Option D is the correct answer. As this is not at all a possible option for the large number of colonies. Presence of antibiotic inhibits the growth so it cannot be the reason.