during the early stages of an enzyme purification protocol, when cells have been
ID: 174267 • Letter: D
Question
during the early stages of an enzyme purification protocol, when cells have been lysed but cytosolic components have not been separated, the reaction velocity versus substrate concentration is sigmoidal. As you continue to purify the enzyme, the curve shifts to the right. Explain your results.
A. this is an enzyme that displays Michaelis-Menten kinentics, and during purification, you remove from solution a homotrophic inhibitor.
B. this is an enzyme that displays Michaelis-Menten kinentics, and during purification, you remove from solution a heterotrophic inhibitor.
C. this is an allosteric enzyme, and during purification, you remove from solution a homotrophic activator.
D. this is an allosteric enzyme, and during purification, you remove from solution a heterotrphic activator.
E. this is an allosteric enzyme, and during purification, you remove from solution a heterotrphic inhibitor.
Explanation / Answer
D This is an allosteric enzyme and during purification you remove from solution a heterotrophic activator.
Enzyme purification is very essential step after the lysis, otherwise it interupts the reaction velocity, so in the mean while reaction velocity and substrate concentration plot gives us many things about Km and reaction time, product concentration. Due allosteric enzyme this shows declining of sigmoidal curve.
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