Reference article \"High-level expression of bioactive recombinant human lysozym

Question

Reference article "High-level expression of bioactive recombinant human lysozyme in the milk of transgenic mice using a modified human lactoferrin BAC" by Shen Liu et al.

Zeocin and neomycin resistances are coded in the recombinant DNAs. Explain the purposes for having these two resistances available for their experiments (don’t just tell me they provide antibiotic resistance) explain the purposes that they serve to advance the experiments- when are they used for selection or to recognize what outcome?).

Explanation / Answer

Expression of bioactivate recombinant human lysozyme in the transgenic mice milk having disease resistance due to lysozymes are catalysing the hydrolysis of 1,4 beta linkage in between N-acetyl-D-glucosamine and N-acetyl muramic acid in the peptidogly which is serve in bacteria cell wall structure, so human lysozyme can causes bacterial cell damages. Zeocin is a glycopeptide antibiotic, therse are breaking the DNA double strand by instering in to DNA and neomycin is a aminoglycoside antibiotic which binds at 30S subunit of bacterial ribosomes interupt the process of protein synthesis. So these 2 antibiotics resistance coding makes helps for elevation of expression of recombinant human lysozymes effects in the experiment.

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